PCR檢測實驗室設計安裝

PCR檢測實驗室設計安裝

PCR實驗室依據所使用的方法可分為試劑準備、標本制備、擴增、產物分析等三或四個區域，各區域應單獨分隔，不應有其他的空氣直通。

試劑準備區儀器設備主要應有加樣器、冰箱、天平、低速離心機、混勻器、可移動紫外燈等，可使用超凈工作臺作為試劑配制操作臺面。標本制備區儀器設備主要應有生物安全柜，為B2，可避免提取核酸在柜內反復循環，造成標本間交叉“污染”，出現假陽性結果；此外還應配備加樣器、臺式高速離心機（冷凍及常溫）、臺式低速離心機、恒溫設備（水浴和/或干浴儀）、冰箱、混勻器和可移動紫外燈等。

PCR增(zeng)加(jia)(jia)區(qu)常見器材只是(shi)核酸PCR增(zeng)加(jia)(jia)熱反復的(de)(de)(de)儀(yi)（PCR儀(yi)，實時(shi)路(lu)況熒光或硬性的(de)(de)(de)）；熱反復的(de)(de)(de)儀(yi)的(de)(de)(de)電流電壓應(ying)專用的(de)(de)(de)，并選(xuan)配(pei)有一(yi)個穩電容式壓或UPS，提防止主(zhu)要(yao)是(shi)因為電流電壓的(de)(de)(de)上下波動對PCR增(zeng)加(jia)(jia)測量的(de)(de)(de)損害；除此之外(wai)只能(neng)根(gen)據本(ben)職工作是(shi)需要(yao)，還可選(xuan)配(pei)加(jia)(jia)樣器、超凈臺等。代謝物淺(qian)析區(qu)：本(ben)區(qu)所適(shi)用的(de)(de)(de)器材系統應(ying)該加(jia)(jia)了樣器、電泳(yong)儀(yi)、電轉(zhuan)印儀(yi)、交配(pei)爐或交配(pei)箱、水浴箱、DNA測序儀(yi)、酶標儀(yi)和洗板(ban)機(ji)等。

PCR科(ke)(ke)學(xue)(xue)進(jin)(jin)行(xing)(xing)試(shi)(shi)驗(yan)報(bao)(bao)(bao)告(gao)(gao)英(ying)(ying)文(wen)(wen)室(shi)內(nei)(nei)(nei)(nei)根(gen)基(ji)公(gong)(gong)用場地設(she)施設(she)計方案：屬(shu)于(yu)科(ke)(ke)學(xue)(xue)進(jin)(jin)行(xing)(xing)試(shi)(shi)驗(yan)報(bao)(bao)(bao)告(gao)(gao)英(ying)(ying)文(wen)(wen)室(shi)內(nei)(nei)(nei)(nei)根(gen)基(ji)房屋門(men)(men)(men)店(dian)裝(zhuang)(zhuang)修、科(ke)(ke)學(xue)(xue)進(jin)(jin)行(xing)(xing)試(shi)(shi)驗(yan)報(bao)(bao)(bao)告(gao)(gao)英(ying)(ying)文(wen)(wen)室(shi)內(nei)(nei)(nei)(nei)油(you)煙(yan)(yan)潔凈(jing)器(qi)(qi)(qi)(qi)模式水(shui)(shui)公(gong)(gong)程(cheng)建設(she)、科(ke)(ke)學(xue)(xue)進(jin)(jin)行(xing)(xing)試(shi)(shi)驗(yan)報(bao)(bao)(bao)告(gao)(gao)英(ying)(ying)文(wen)(wen)室(shi)內(nei)(nei)(nei)(nei)供排(pai)出污水(shui)(shui)系(xi)(xi)(xi)統(tong)(tong)公(gong)(gong)程(cheng)建設(she)、科(ke)(ke)學(xue)(xue)進(jin)(jin)行(xing)(xing)試(shi)(shi)驗(yan)報(bao)(bao)(bao)告(gao)(gao)英(ying)(ying)文(wen)(wen)室(shi)內(nei)(nei)(nei)(nei)電(dian)(dian)(dian)源(yuan)電(dian)(dian)(dian)路系(xi)(xi)(xi)統(tong)(tong)系(xi)(xi)(xi)統(tong)(tong)。科(ke)(ke)學(xue)(xue)進(jin)(jin)行(xing)(xing)試(shi)(shi)驗(yan)報(bao)(bao)(bao)告(gao)(gao)英(ying)(ying)文(wen)(wen)室(shi)內(nei)(nei)(nei)(nei)根(gen)基(ji)房屋門(men)(men)(men)店(dian)裝(zhuang)(zhuang)修要有干凈(jing)家裝(zhuang)(zhuang)吊頂、地面、隔(ge)，合理可行(xing)(xing)放置(zhi)(zhi)臨時文(wen)(wen)件間及搭配油(you)煙(yan)(yan)潔凈(jing)器(qi)(qi)(qi)(qi)模式水(shui)(shui)門(men)(men)(men)、觀擦窗、傳(chuan)播窗等(deng)；科(ke)(ke)學(xue)(xue)進(jin)(jin)行(xing)(xing)試(shi)(shi)驗(yan)報(bao)(bao)(bao)告(gao)(gao)英(ying)(ying)文(wen)(wen)室(shi)內(nei)(nei)(nei)(nei)油(you)煙(yan)(yan)潔凈(jing)器(qi)(qi)(qi)(qi)模式水(shui)(shui)公(gong)(gong)程(cheng)建設(she)應(ying)標(biao)準(zhun)配置(zhi)(zhi)單隨便(bian)送通風口公(gong)(gong)用場地設(she)施，化學(xue)(xue)制劑搭配區(qu)、核酸拆分(fen)區(qu)和(he)擴(kuo)張(zhang)了(le)解區(qu)應(ying)標(biao)準(zhun)配置(zhi)(zhi)單油(you)煙(yan)(yan)潔凈(jing)器(qi)(qi)(qi)(qi)模式水(shui)(shui)系(xi)(xi)(xi)統(tong)(tong)；科(ke)(ke)學(xue)(xue)進(jin)(jin)行(xing)(xing)試(shi)(shi)驗(yan)報(bao)(bao)(bao)告(gao)(gao)英(ying)(ying)文(wen)(wen)室(shi)內(nei)(nei)(nei)(nei)供排(pai)出污水(shui)(shui)系(xi)(xi)(xi)統(tong)(tong)公(gong)(gong)程(cheng)建設(she)屬(shu)于(yu)非自(zi)動改(gai)手動水(shui)(shui)領(ling)先(xian)、不銹鋼裝(zhuang)(zhuang)飾管(guan)(guan)嗎(ma)或PP水(shui)(shui)池(chi)子和(he)及時淋浴(yu)花灑器(qi)(qi)(qi)(qi)及供排(pai)出污水(shui)(shui)系(xi)(xi)(xi)統(tong)(tong)輸(shu)水(shui)(shui)管(guan)(guan)線聚氨酯保(bao)溫管(guan)(guan)；科(ke)(ke)學(xue)(xue)進(jin)(jin)行(xing)(xing)試(shi)(shi)驗(yan)報(bao)(bao)(bao)告(gao)(gao)英(ying)(ying)文(wen)(wen)室(shi)內(nei)(nei)(nei)(nei)電(dian)(dian)(dian)源(yuan)電(dian)(dian)(dian)路系(xi)(xi)(xi)統(tong)(tong)系(xi)(xi)(xi)統(tong)(tong):要根(gen)據機(ji)器(qi)(qi)(qi)(qi)系(xi)(xi)(xi)統(tong)(tong)的(de)(de)(de)工(gong)(gong)作效(xiao)(xiao)率(lv)搭配適合的(de)(de)(de)工(gong)(gong)作效(xiao)(xiao)率(lv)，需(xu)關注(zhu)高電(dian)(dian)(dian)壓殺菌鍋的(de)(de)(de)工(gong)(gong)作效(xiao)(xiao)率(lv)，需(xu)關注(zhu)科(ke)(ke)學(xue)(xue)進(jin)(jin)行(xing)(xing)試(shi)(shi)驗(yan)報(bao)(bao)(bao)告(gao)(gao)英(ying)(ying)文(wen)(wen)室(shi)內(nei)(nei)(nei)(nei)耗電(dian)(dian)(dian)安全性高；標(biao)準(zhun)配置(zhi)(zhi)單支(zhi)持的(de)(de)(de)救急(ji)指揮儀器(qi)(qi)(qi)(qi)，如救急(ji)指揮照明系(xi)(xi)(xi)統(tong)(tong)系(xi)(xi)(xi)統(tong)(tong)、消防安全儀器(qi)(qi)(qi)(qi)、意外死亡死亡事故治(zhi)療儀器(qi)(qi)(qi)(qi)等(deng)。

化學試劑做準備區

該檢測區基(ji)本參(can)與(yu)的操作流程為儲放微生(sheng)物(wu)培(pei)(pei)養基(ji)的制(zhi)法、微生(sheng)物(wu)培(pei)(pei)養基(ji)的預包(bao)裝和主體現混合(he)型液的制(zhi)法。

微生物(wu)(wu)培(pei)養(yang)基(ji)和于樣本設計(ji)制(zhi)作的(de)(de)建材(cai)(cai)應(ying)隨時長途(tu)運輸至該地，不(bu)應(ying)過程其(qi)它區域(yu)環境。微生物(wu)(wu)培(pei)養(yang)基(ji)原(yuan)建材(cai)(cai)低(di)溫干(gan)燥在本消防通道，并在本消防通道提純成營養(yang)的(de)(de)低(di)溫干(gan)燥微生物(wu)(wu)培(pei)養(yang)基(ji)。

實驗試劑須(xu)要準備區裝(zhuang)備有(you)2~8°C墻上(shang)(shang)和-80°C墻上(shang)(shang)，算方式冷供(gong)電量(liang)時，須(xu)要將兩者算方式以外。

房子的(de)范圍(wei)宜調控(kong)在15m2~20m2。本區(qu)的(de)負荷均值標準要(yao)求為:相對來說+壓(ya)的(de)情(qing)況，防患未然止外力(li)含核酸氣溶膠的(de)氣氛邁入，容易造成(cheng)環(huan)保(bao)問題。